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COVID-19 Vaccines: All Transfection Methods will use (Luciferase Gene)



All Transfection Methods will use (Luciferase Gene)


3 Methods of Transfection


1) Lipid Based Transfection

2) Calcium Phosphate

3) Electroporation (Use of Electricity)


1st Transfection Method: Lipid based


Transfection Method: Lipid (Luciferase Gene)


Enhanced in vivo bioluminescence imaging using liposomal luciferin delivery system


https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2815139/


A Potent Branched-Tail Lipid Nanoparticle Enables Multiplexed mRNA Delivery and Gene Editing In Vivo


https://pubs.acs.org/doi/10.1021/acs.nanolett.0c00596


Quantum Dots & Nanoparticles


Functionalized lipid-like nanoparticles for in vivo mRNA delivery and base editing


https://advances.sciencemag.org/content/6/34/eabc2315


Lipid Nanoparticles for Gene Delivery


https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006671/


Lipid-mRNA Nanoparticle Designed to Enhance Intracellular Delivery Mediated by Shock Waves


https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6602409/


2nd Transfection Method: Calcium Phosphate


Transfection Method: Calcium Phosphate (Luciferase Gene)


Non-Invasive In Vivo Imaging of Calcium Signaling in Mice


https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1991622/


Transfection of Luciferase with Delivery System of Nanoparticles


Calcium phosphate nanoparticles-based systems for siRNA delivery


https://academic.oup.com/rb/article/3/3/187/1752885


Quantum Dots & Nanoparticles


Preliminary in vivo magnetofection data using magnetic calcium phosphate nanoparticles immobilizing DNA and iron oxide nanocrystals


https://www.sciencedirect.com/science/article/pii/S2352340918304116


Biodegradable Calcium Phosphate Nanoparticle with Lipid Coating for Systemic siRNA Delivery


https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2833237/


3rd Transfection Method: Electroporation


Transfection Method: Electroporation (Luciferase Gene)


Electroporation as a method for high-level nonviral gene transfer to the lung


https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5576138/


In vivo electroporation and ubiquitin promoter--a protocol for sustained gene expression in the lung


https://pubmed.ncbi.nlm.nih.gov/16685743/


A Critical Review of Electroporation as A Plasmid Delivery System in Mouse Skeletal Muscle


https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600476/


Visualization of in vivo electroporation mediated transgene expression in experimental tumors by optical and magnetic resonance imaging


https://www.nature.com/articles/gt200955


Electroporation of Mammalian Cells with a Firefly Luciferase Expression Plasmid: Kinetics of Transient Expression Differ Markedly among Cell Types


https://www.liebertpub.com/doi/10.1089/dna.1.1988.7.557


Quantum Dots & Nanoparticles


Gold Nanoparticles Enhanced Electroporation for Mammalian Cell Transfection


https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696415/


Electroporation for nanomedicine: a review


https://pubs.rsc.org/en/content/articlelanding/2017/tb/c7tb00038c#!divAbstract


Electroporation of a nanoparticle-associated DNA vaccine induces higher inflammation and immunity compared to its delivery with microneedle patches in pigs


https://www.sciencedirect.com/science/article/pii/S0168365919303840


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