COVID-19 Vaccines: All Transfection Methods will use (Luciferase Gene)
All Transfection Methods will use (Luciferase Gene)
3 Methods of Transfection
1) Lipid Based Transfection
2) Calcium Phosphate
3) Electroporation (Use of Electricity)
1st Transfection Method: Lipid based
Transfection Method: Lipid (Luciferase Gene)
Enhanced in vivo bioluminescence imaging using liposomal luciferin delivery system
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2815139/
A Potent Branched-Tail Lipid Nanoparticle Enables Multiplexed mRNA Delivery and Gene Editing In Vivo
https://pubs.acs.org/doi/10.1021/acs.nanolett.0c00596
Quantum Dots & Nanoparticles
Functionalized lipid-like nanoparticles for in vivo mRNA delivery and base editing
https://advances.sciencemag.org/content/6/34/eabc2315
Lipid Nanoparticles for Gene Delivery
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006671/
Lipid-mRNA Nanoparticle Designed to Enhance Intracellular Delivery Mediated by Shock Waves
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6602409/
2nd Transfection Method: Calcium Phosphate
Transfection Method: Calcium Phosphate (Luciferase Gene)
Non-Invasive In Vivo Imaging of Calcium Signaling in Mice
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1991622/
Transfection of Luciferase with Delivery System of Nanoparticles
Calcium phosphate nanoparticles-based systems for siRNA delivery
https://academic.oup.com/rb/article/3/3/187/1752885
Quantum Dots & Nanoparticles
Preliminary in vivo magnetofection data using magnetic calcium phosphate nanoparticles immobilizing DNA and iron oxide nanocrystals
https://www.sciencedirect.com/science/article/pii/S2352340918304116
Biodegradable Calcium Phosphate Nanoparticle with Lipid Coating for Systemic siRNA Delivery
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2833237/
3rd Transfection Method: Electroporation
Transfection Method: Electroporation (Luciferase Gene)
Electroporation as a method for high-level nonviral gene transfer to the lung
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5576138/
In vivo electroporation and ubiquitin promoter--a protocol for sustained gene expression in the lung
https://pubmed.ncbi.nlm.nih.gov/16685743/
A Critical Review of Electroporation as A Plasmid Delivery System in Mouse Skeletal Muscle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600476/
Visualization of in vivo electroporation mediated transgene expression in experimental tumors by optical and magnetic resonance imaging
https://www.nature.com/articles/gt200955
Electroporation of Mammalian Cells with a Firefly Luciferase Expression Plasmid: Kinetics of Transient Expression Differ Markedly among Cell Types
https://www.liebertpub.com/doi/10.1089/dna.1.1988.7.557
Quantum Dots & Nanoparticles
Gold Nanoparticles Enhanced Electroporation for Mammalian Cell Transfection
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696415/
Electroporation for nanomedicine: a review
https://pubs.rsc.org/en/content/articlelanding/2017/tb/c7tb00038c#!divAbstract
Electroporation of a nanoparticle-associated DNA vaccine induces higher inflammation and immunity compared to its delivery with microneedle patches in pigs
https://www.sciencedirect.com/science/article/pii/S0168365919303840