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COVID-19 Vaccines: All Transfection Methods will use (Luciferase Gene)

All Transfection Methods will use (Luciferase Gene)

3 Methods of Transfection

1) Lipid Based Transfection

2) Calcium Phosphate

3) Electroporation (Use of Electricity)

1st Transfection Method: Lipid based

Transfection Method: Lipid (Luciferase Gene)

Enhanced in vivo bioluminescence imaging using liposomal luciferin delivery system

A Potent Branched-Tail Lipid Nanoparticle Enables Multiplexed mRNA Delivery and Gene Editing In Vivo

Quantum Dots & Nanoparticles

Functionalized lipid-like nanoparticles for in vivo mRNA delivery and base editing

Lipid Nanoparticles for Gene Delivery

Lipid-mRNA Nanoparticle Designed to Enhance Intracellular Delivery Mediated by Shock Waves

2nd Transfection Method: Calcium Phosphate

Transfection Method: Calcium Phosphate (Luciferase Gene)

Non-Invasive In Vivo Imaging of Calcium Signaling in Mice

Transfection of Luciferase with Delivery System of Nanoparticles

Calcium phosphate nanoparticles-based systems for siRNA delivery

Quantum Dots & Nanoparticles

Preliminary in vivo magnetofection data using magnetic calcium phosphate nanoparticles immobilizing DNA and iron oxide nanocrystals

Biodegradable Calcium Phosphate Nanoparticle with Lipid Coating for Systemic siRNA Delivery

3rd Transfection Method: Electroporation

Transfection Method: Electroporation (Luciferase Gene)

Electroporation as a method for high-level nonviral gene transfer to the lung

In vivo electroporation and ubiquitin promoter--a protocol for sustained gene expression in the lung

A Critical Review of Electroporation as A Plasmid Delivery System in Mouse Skeletal Muscle

Visualization of in vivo electroporation mediated transgene expression in experimental tumors by optical and magnetic resonance imaging

Electroporation of Mammalian Cells with a Firefly Luciferase Expression Plasmid: Kinetics of Transient Expression Differ Markedly among Cell Types

Quantum Dots & Nanoparticles

Gold Nanoparticles Enhanced Electroporation for Mammalian Cell Transfection

Electroporation for nanomedicine: a review!divAbstract

Electroporation of a nanoparticle-associated DNA vaccine induces higher inflammation and immunity compared to its delivery with microneedle patches in pigs

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