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COVID Diagnosis with PCR | Misinterpreting results | Cycle threshold explained


***Links to the underlying research and articles referenced in this video below:


Duration of infectiousness and correlation with RT-PCR cycle threshold values in cases of COVID-19, England, January to May 2020 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7427302/


63 days detection of SARS-CoV-2 RNA from a recovered patient in Southern Italy: A Case Report | http://www.seu-roma.it/riviste/annali_igiene/open_access/articoli/32-05-11-Baldacci.pdf


Absent immune response to SARS-CoV-2 in a 3-month recurrence of coronavirus disease 2019 (COVID-19) case | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7386381/


SARS-CoV-2 RNA Persistence in Naso-Pharyngeal Swabs | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466010/


Long-Haulers Are Redefining COVID-19 | https://www.theatlantic.com/health/archive/2020/08/long-haulers-covid-19-recognition-support-groups-symptoms/615382/


COVID-19 re-infection by a phylogenetically distinct SARS-coronavirus-2 strain confirmed by whole genome sequencing | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7499500/


To Interpret the SARS-CoV-2 Test, Consider the Cycle Threshold Value | https://academic.oup.com/cid/article/71/16/2252/5841456


Your Coronavirus Test Is Positive. Maybe It Shouldn’t Be. | https://www.nytimes.com/2020/08/29/health/coronavirus-testing.html


Testing Dilemmas: Post negative, positive SARS-CoV-2 RT-PCR - is it a reinfection? |

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7228728/


Challenges and Controversies to Testing for COVID-19 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7587118/


‘It’s Kitchen Sink Time’: Fast, Less-Accurate Coronavirus Tests May Be Good Enough |

https://www.nytimes.com/2020/08/06/health/rapid-Covid-tests.html


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****By popular demand, isolation and purification of SARS-CoV-2 and fulfillment of Koch's postulates (keep in mind, though, that Koch made his postulates to describe cholera and TB and was written in the 1800s before viruses were discovered): What does it mean to isolate and purify a virus? http://eacharya.inflibnet.ac.in/data-server/eacharya-documents/55d44ff9e41301fd23d8facc_INFIEP_203/660/ET/203-660-ET-V1-S1__lec_6.pdf


A virologist's summary and review of relevant literature:

https://virologydownunder.com/sigh-yes-the-covid-virus-is-real/


The pathogenicity of SARS-CoV-2 in hACE2 transgenic mice (fulfillment of Koch's postulates) https://www.nature.com/articles/s41586-020-2312-y


Isolation, Sequence, Infectivity, and Replication Kinetics of Severe Acute Respiratory Syndrome Coronavirus 2 | https://wwwnc.cdc.gov/eid/article/26/9/20-1495_article

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Virus Isolation from the First Patient with SARS-CoV-2 in Korea | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036342/


Isolation and characterization of SARS-CoV-2 from the first US COVID-19 patient |

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7239045/


SARS-CoV-2 infection diagnosed only by cell culture isolation before the local outbreak in an Italian seven-week-old suckling baby | https://www.sciencedirect.com/science/article/pii/S1201971220303428


Isolation of infectious SARS-CoV-2 from urine of a COVID-19 patient |

https://www.tandfonline.com/doi/full/10.1080/22221751.2020.1760144



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