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COVID Diagnosis with PCR | Misinterpreting results | Cycle threshold explained

***Links to the underlying research and articles referenced in this video below:

Duration of infectiousness and correlation with RT-PCR cycle threshold values in cases of COVID-19, England, January to May 2020 |

63 days detection of SARS-CoV-2 RNA from a recovered patient in Southern Italy: A Case Report |

Absent immune response to SARS-CoV-2 in a 3-month recurrence of coronavirus disease 2019 (COVID-19) case |

SARS-CoV-2 RNA Persistence in Naso-Pharyngeal Swabs |

Long-Haulers Are Redefining COVID-19 |

COVID-19 re-infection by a phylogenetically distinct SARS-coronavirus-2 strain confirmed by whole genome sequencing |

To Interpret the SARS-CoV-2 Test, Consider the Cycle Threshold Value |

Your Coronavirus Test Is Positive. Maybe It Shouldn’t Be. |

Testing Dilemmas: Post negative, positive SARS-CoV-2 RT-PCR - is it a reinfection? |

Challenges and Controversies to Testing for COVID-19 |

‘It’s Kitchen Sink Time’: Fast, Less-Accurate Coronavirus Tests May Be Good Enough |


****By popular demand, isolation and purification of SARS-CoV-2 and fulfillment of Koch's postulates (keep in mind, though, that Koch made his postulates to describe cholera and TB and was written in the 1800s before viruses were discovered): What does it mean to isolate and purify a virus?

A virologist's summary and review of relevant literature:

The pathogenicity of SARS-CoV-2 in hACE2 transgenic mice (fulfillment of Koch's postulates)

Isolation, Sequence, Infectivity, and Replication Kinetics of Severe Acute Respiratory Syndrome Coronavirus 2 |


Virus Isolation from the First Patient with SARS-CoV-2 in Korea |

Isolation and characterization of SARS-CoV-2 from the first US COVID-19 patient |

SARS-CoV-2 infection diagnosed only by cell culture isolation before the local outbreak in an Italian seven-week-old suckling baby |

Isolation of infectious SARS-CoV-2 from urine of a COVID-19 patient |

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