COVID Diagnosis with PCR | Misinterpreting results | Cycle threshold explained

***Links to the underlying research and articles referenced in this video below:

Duration of infectiousness and correlation with RT-PCR cycle threshold values in cases of COVID-19, England, January to May 2020 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7427302/

63 days detection of SARS-CoV-2 RNA from a recovered patient in Southern Italy: A Case Report | http://www.seu-roma.it/riviste/annali_igiene/open_access/articoli/32-05-11-Baldacci.pdf

Absent immune response to SARS-CoV-2 in a 3-month recurrence of coronavirus disease 2019 (COVID-19) case | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7386381/

SARS-CoV-2 RNA Persistence in Naso-Pharyngeal Swabs | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466010/

COVID-19 re-infection by a phylogenetically distinct SARS-coronavirus-2 strain confirmed by whole genome sequencing | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7499500/

To Interpret the SARS-CoV-2 Test, Consider the Cycle Threshold Value | https://academic.oup.com/cid/article/71/16/2252/5841456

Testing Dilemmas: Post negative, positive SARS-CoV-2 RT-PCR - is it a reinfection? |

Challenges and Controversies to Testing for COVID-19 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7587118/

‘It’s Kitchen Sink Time’: Fast, Less-Accurate Coronavirus Tests May Be Good Enough |

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****By popular demand, isolation and purification of SARS-CoV-2 and fulfillment of Koch's postulates (keep in mind, though, that Koch made his postulates to describe cholera and TB and was written in the 1800s before viruses were discovered): What does it mean to isolate and purify a virus? http://eacharya.inflibnet.ac.in/data-server/eacharya-documents/55d44ff9e41301fd23d8facc_INFIEP_203/660/ET/203-660-ET-V1-S1__lec_6.pdf

A virologist's summary and review of relevant literature:

The pathogenicity of SARS-CoV-2 in hACE2 transgenic mice (fulfillment of Koch's postulates) https://www.nature.com/articles/s41586-020-2312-y

Isolation, Sequence, Infectivity, and Replication Kinetics of Severe Acute Respiratory Syndrome Coronavirus 2 | https://wwwnc.cdc.gov/eid/article/26/9/20-1495_article

Virus Isolation from the First Patient with SARS-CoV-2 in Korea | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036342/

Isolation and characterization of SARS-CoV-2 from the first US COVID-19 patient |

SARS-CoV-2 infection diagnosed only by cell culture isolation before the local outbreak in an Italian seven-week-old suckling baby | https://www.sciencedirect.com/science/article/pii/S1201971220303428

Isolation of infectious SARS-CoV-2 from urine of a COVID-19 patient |

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